Monday, February 18, 2008

Dragon Lab and Punnet Square Lab


This is the punnet square of the Flys!


This is the Dragons!



The first lab we are doing is on the dragons, with this lab we are using different recessive and dominate genes to help the dragons become similar. This will include changed the color, shape, and facial features different of one dragon to have it look similar to the other.
The second lab we are going to use punnett squares to see who will have the long wings when they are born. Each parent has one recessive and dominate gene. This gives there kids one in 4 chances to have short wings.

These are showing how we inherent different traits. When a baby is conceived they receive 23 chromosomes from each parent. With in these chromosomes the child will there gender chosen, skin color, blue or brown eyes, hair color, and so on.
The genotype is the genes of an individual for a particular trait or traits; often designated by letters, BB or Bb. When you are in the dragon lab and you are trying to get the two dragons to match you choosing different recessive and dominate genes to change the color, horn, feet, and other characteristics.
Phenotype is the visible results of the genotype. This shown in the dragon lab by the color of the wings, horn and the visible characteristics.
Allele is the alternative for of a gene.
The dominant genes mask the expression of the recessive gene.
The recessive genes this takes a back seat to a dominate gene.

During the lab you are using dominate and recessive genes to help determine the different characteristics of the fruit-fly’s and dragons. When there is a dominate gene involved the recessive gene will always loose.
However there is an exception the recessive gene. The recessive gene will be shown if there are two recessive genes involved.
As you can see the dragon has an Hh gene to create the horns on the dragon. If we were to put two recessive genes there, their wouldn’t be any horns. This how the dominate and recessive gene are shown in this lab. Amongst the other characteristics.
Considering that both parents are recessive and dominate then each of there children will also have the possibility of having the recessive gene of short wings. This means that if they have babies with another fruit-fly and they have the recessive gene then there kids will have the possibility of having the short wings too.

Com 18-21

Chapter 18 Patterns of Chromosome in heritance

18.1 Chromosome and the cell cycle

Humans carry 46 chromosomes at all times these are in 23 pairs. 22 of these are autosomes and 1 pair of these is the sex chromosomes. Males have and X and Y. The females have two X chromosomes.

The Cell Cycle

The cell cycle is an orderly process that has two parts: interphase and cell division.
· The interphase is where the cell spends most its time.
o G1 stage: The cell doubles it organelles and it accumulates the materials need for DNA synthesis.
o S stage the DNA replication occurs.
o G2 stage the cell synthesizes the proteins need for cell division.

· Cell Division
o Mitotic stage distributes two sister chromosomes into the cell
o Cytokinesis is the division of the cytoplasm,

18.2 Mitosis
Prophase
Prophase

o The chromosomes condense and are visible.
o Nuclear envelope fragment
o The nucleus disappears.
o The centrosomes to opposite sides
o The spindle fibers appear

Metaphase

Metaphase

o The chromosomes then line up in the middle of the cell,
o The spindle is now fully formed.

Anaphase

o The sister chromatics separate at the middle and start to move towards the “poles”
This a picture showing the telophase

The telophase and Cytokinesis

o The chromosomes finally make it to there destination.
o The nuclei reappears
o The spindle disappears
o The nuclear envelope is put back together again
o This all works towards making two identical daughter cells.

18.3 Meiosis
Prophase 1

Prophase 1

o Meiosis is part of the sexual reproduction
o Synapsis occurs
o The spindle forms
o The nuclear envelope fragments
o During synapsis homologous chromosomes come together and line up side by side.
o An exchange of genetic material may occur now “Crossing Over”

Metaphase 1

o Homologous pairs align independently at the equator.

18.4 Comparison of Meiosis and Mitosis

o Homologous chromosomes pair and undergo crossing over during prophase 1 or meiosis but not during mitosis
o Paired homologous chromosomes align at the equator during metaphase 1 in meiosis. These paired chromosomes have 4 chromitids altogether individual chromosomes align at the equator during metaphase in mitosis. They each have two chromatids.
o Meiosis 2 and mitosis is the same except that the nuclei contain the haploid number of chromosomes.

Spermatogenesis and Oogenesis

The spermatogenesis is the production of sperm in males. After the sexual organs become mature the average male will produce 300,000 a minute, (400 mil a day) even though there are so many only a fractions will ever get the chance to fertilize an egg.
Oogenesis is the production of eggs in the female. A mature egg has 23 chromosomes.

18.5 Chromosome Inheritance

if an egg has 24 chromosomes rather then 23 then trisomy occurs. If the egg has 22 rather then 23 then monosomy occurs. These will both create a Barr Body. When an extra Y is present then Jacobs Syndrome occurs. Down syndrome other wise know as trisomy 21. Someone born with only one sex chromosome then its Turner syndrome. Klinefelter happens in about 1 in 650 males, they are born with two X chromosomes and a Y chromosome. Only about 25% of males that have it will ever be diagnosed.

Chapter 19 Cancer

19.1 Cancer Cells

Cancer cells lack differentiation
They have an abnormal nuclei (they are enlarged and have an abnormal of chromosomes)
They have unlimited replicate potential.
They form tumors
Cancer cells have no need for growth factors
Cancer cells gradually become abnormal
Through Carinogenesis
Initiation: a single cell undergoes a mutation that causes it to begin to divide repeatedly
Promotion: a tumor develops and the tumor cells continue to divide. As they divide they undergo mutation
Progression: one cell undergoes a mutation that gives it a selective advantage over the other cells. This process is repeated several times and eventually there is a cell that has the ability to invade surrounding tissues.
§ They undergo Angiogenesis and Metastasis

Cancer is a genetic Disease

1. Proto-oncogenes: is the code for proteins that promote the cell cycle and prevent apoptosis, they are often likened to the gas pedal of a car because they cause acceleration.
2. Tumor-suppressor genes: code for proteins that inhibit the cell cycle and promote apoptosis, they are often likened to the brakes of the car because they inhibit acceleration

Types of cancer

A patient’s prognosis is determined by two different stages:
1. whether the tumor has invaded the surrounding tissue
2. whether there are metastatic tumors in distant parts of the body

Prostate cancer

Classification of tumors:
1. Carcinomas: cancers of the epithelial tissues and Aden-carcinomas are tumors of the glandular cells. This includes:
a. Skin
b. Breast
c. Liver
d. Pancreas
e. Intestines
f. Lung
g. Prostate
h. Thyroid
2. Sarcomas are cancers that arise in the muscles and connective tissue. Such as bone and fibrous connective tissue.
3. Leukemia’s are cancers of the blood
4. Lymphomas are cancers of the lymphatic system

Breast cancer is the most common type of cancer that is found in the body. (Mainly in women) For males the most common type of cancer is prostate cancer.
Skin Cancer

19.2 Causes and Prevention of Cancer

The causes of cancer are genetics and environmental carcinogens
§ Radiation
o UV lights, radon gas, nuclear fuel, and X-ray.
§ These are responsible for:
· Skin Cancer
§ Organic Chemicals
o Such as tobacco smoke
§ These contain chemicals that can cause mutations:
· Larynx
· Esophagus
· Pancreas
· Bladder
· Kidney
· cervix
o Pollutants
§ Chemicals and pesticides
· Lung disease
· Lung cancer
o And Viruses
§ Hep B an C
Epstein-Barr

§ Epstein-Barr Virus
§ HPV
· Liver cancer
· Cervical Cancer
· Nasopharyngeal cancer

§ Dietary choices have also shown evidence that it can play a role in it.
o Such as high fat content:
§ Breast and prostate cancer

19.3 Diagnosis of Cancer

There are seven warning signs:
1. C- Change in bowel or bladder habits
2. A- a sore that does not heal
3. U- unusual bleeding or discharge
4. T- Thickening or lump in breast or elsewhere
5. I- indigestion or difficulty in swallowing
6. O- Obvious change in wart or mole
7. N- Nagging cough or hoarseness

Routine screening tests:
Self-Examination tests:
1. ABCD’s of melanoma
a. Asymmetry
b. Border, irregular scalloped or poorly circumscribed border.
c. Color, Varied from one area to another. Shades of tan, brown, black, red, white, and blue.
d. Diameter, larger then 6mm
2. Mammogram
3. Pap smear
4. Colonoscopy

Tumor marker tests are a blood test for tumor antigens and antibodies.
Genetic tests for mutations in proto-oncogenes and tumor suppressor. These are available for breast, colon, bladder, thyroid, and melanoma.

19.4 Treatment of Cancer

The standard therapies:
Surgery
Radiation
Chemo
Bone marrow transplants
Newer treatments:
Immunotherapy
Passive immunotherapy
P53 gene
Angiogenesis

Chapter 20 Patterns of Genetic Inheritance

20.1 Genotype and Phenotype
The genotype of a human refers to the genes of an individual,

20.2 One and Two trait Inheritance

One and two trait inheritance has to do with the recessive and dominate traits. Each trait has a link between being recessive which means that it will take two recessive genes of each parent to pass it on to there children, some others are dominate and need only one to pass it on to there children.

Different genetic disorders those are recessive:
a. Tay- sachs
b. Phenylketonuria
c. Sickle cell
Different genetic disorders that are dominate:
a. Marfan syndrome
b. Huntington disease

20.4 Sex-linked Inheritance

Autosomes are the 22 chromsomes out of the 23 chromosomes. The 1 chromosome that is left is the sex chromosomes these determine the sex of the human. The traits that are from the genes of the sex chromosomes are said to be sex linked. The allele of the x chromosome is X-linked. And the same for the Y.

Chapter 21 DNA Biology and Technology

21.1 DNA and RNA structure and Function

The structure of DNA is double helix.

Replication of DNA

When an exact copy is made of the DNA helix then it is considered to be DNA replication.
1. Before replication begins the two strands that make up the parental DNA are hydrogen bonded to each other.\
2. an enzyme unwinds and unzips double stranded DNA
3. New complementary DNA nucleotides, always present in the nucleus, fit into place by the process of complementary base pairing. These are positioned and joined by the enzyme DNA polymerase
4. To complete replication an enzyme seals any breaks in the sugar phosphate backbone.
5. The two double helix molecules are identical to each other and to the original DNA molecule.

The structure and replication of RNA

RNA is single stranded and is translated unlike DNA that is transcribed.

21.2 Gene Expression

Transcription
Happens in the Nucleus
DNA double helix opens up
mRNA transcript is made from the DNA template
Translation
Happens outside the nucleus
ribosome’s do the translation
they glom onto the mRNA and line up the acids according to he mRNA code
The regulation of gene expression

1. Transcriptional control
a. Chromatin density and transcription control
2. posttranscriptional control
a. mRNA processing
3. translational code
a. Differential ability of mRNA due to ribosome’s
4. post translation code
a. changes to the protein to make it functional
When the regulation of a gene is out of control then cancer takes its place.

21.3 Genomics

proteomics is the study of the structure, function, and interaction of cellular proteins.

Bioinformatics is the application of computer technologies to the study of the genome.

This can be modified through
1. ex vivo gene therapy
a. bone marrow stem cells are removed and infected with the RNA retrovirus
b. this carries the normal gene
c. Then returned to the patient.
2. in vivo gene therapy:
a. the gene needed to cure cystic fibrosis is sprayed up the nose
b. or delivered to the lower respiratory tract by adeno viruses

21.4 DNA technology

Recombinant DNA
DNA sequencing and human genome project
Genetic engineering

These are the websites that I was able to get some information off of:

http://virtual.yosemite.cc.ca.us/randerson/Lynn

library.thinkquest.org/.../mitosis.gif

http://micro.magnet.fsu.edu/cells/fluorescencemitosis/images/cytokinesissmall.jpg

www.lib.uiowa.edu/hardin/md/pictures22/dermne...

http://www.breasthealthfocus.com/articles/images/imgCancerSymptoms.gif

http://www.meb.uni-bonn.de/cancer.gov/Media/CDR0000442273.jpg

commons.wikimedia.org/wiki/Image:Epstein_Barr...

http://www.dartmouth.edu/~toxmetal/images/sicklecell.jpg

http://www.allaboutarthritis.com/image/stock_image/marfan_syndrome02.jpg

www.ch.cam.ac.uk/.../molecules/nucleic/dna1.jpg

Com Chapters 1-4














































1.1 Characteristics of life

Life is organized

There are many different characteristics of life. We can categorize them making them less confusing. Starting with life is organized; this consists from atoms through the biosphere. Please view picture 1. This will explain each level of life.

Acquiring materials and energy

Humans and animals cannot function with out energy. We get our energy from the food that we eat that contains nutrients. The nutrients are then broken down to help store energy with in our bodies.

Reproducing

The human race reproduces to ensure that we will be around for centuries to come. During the DNA reproduction it sends information into a cell and to it metabolism. Then DNA makes exact copies of it self, Genes that are later passed onto the offspring.

Growing and Developing

We are growing from the moment that we were conceived until death has taken its place. In uterus we are growing rapidly making the different organs, tissues, and so on. We will never stop growing physically or mentally.

Homeostasis

Being homeostatic we maintain our body temperature. We are also using the digestive system to take in nutrients while other organs and areas of the body are taking the nutrients and dispensing them where needed. Thus giving is energy.

Responding to stimuli

As humans we can respond to different stimulus. Some humans are moved by the sight of food others by the perfect pair of shoes in the window. When you touch a hot stove you will have something go off in your body saying that it is hot and that it needs to be removed, this will happen simultaneously.

Life has an evolutionary history

-Evolution is the process by which a species changes through time.
Each generation changes a little to help suit them for the environmental changes and the physical demands.

1.2 Humans are related to other animals

All living things are classified under 3 domain groups.
1. Domain Eurkarya
a. Kingdom animalia
b. Kingdom plants
c. Kingdom fungi
d. Kingdom protista
2. Domain archaea
3. Bacteria

Humans are classified as vertebrates in kingdom animalia. We are distinguished by our highly developed brain, completely upright position, creative language, and have the ability to use a large variety of tools.

Humans have a cultural heritage

This engulfs us with products that are handed down from generation to generation. When we are born it is like a blank slate and our cultural surrounding are what mold us to be who we are.

Humans are members of the biosphere

All living things are categorized as part of the biosphere. This includes the soil to the atmosphere. We depend on the environment to shelter, feed, warm, and give us oxygen.

Humans threaten the biosphere

We cut the forest down to nothing and we have cars that are eating away at the atmosphere slowing causing global warming. When we cut down the forests we are also taking down animal’s shelters and food supply thus killing most species to extinction.

1.3 Science as a process

Importance of scientific theories

The ultimate gold of science is to understand the natural world in terms of scientific theories. Scientific theories tell us how life is organized.

The scientific method has steps

1. Observation- new observations are made and previous data are studied
2. Hypothesis- input from various sources is used to formulate a testable statement.
3. Experiment/ observations- the hypothesis is tested by experiment or further observations
4. conclusion- the results are analyzed and the hypothesis is supported or rejected
5. Scientific theory- many experiments and observations support a theory.

How the cause of ulcers was discovered

First there was bacteria suspected and was able to isolate the Heliobacter Phylori from the ulcer patients. Then tried to infect a guinea pig and rats. This didn’t work then he decided that he would be the test dummy and drank a whole vial of the bacteria and come to find out that is what it was.

How to conduct a control study

There needs to be three groups of people. One would be given the placebo, one the first antibiotic, and the third group the second antibiotic. After a certain amount of time each of the groups are pulled in to the results. When this taken place is might be a double blind procedure. This means that the doctors checking them out wont know who got what only making it fair to not make there own statements.

Scientific journals versus other sources of information

Scientific journals are written by scientist and are not in lemons terms making them difficult to read and understand. However they are the most reliable for accurate information on a specific study. When you are getting through a magazine or other source they may alter the information a little and not give the full story.

1.4 making sense of the scientific study

There are many different aspects that you should look for when researching a study. One is methodology this explains how the study was done. The standard error is the second thing you should look for because it shows how uncertain a particular value is.

1.5 Science and social responsibility

Science technology has always believe that it can offer ways to improve and advance the civilization by being able to improve paving, crops, and curing diseases.

Science and technology, benefits versus risks

GM crops were built to help diabetic patients have insulin but even though there are benefits there are risks. Some of these risks may include the toxin that is being grown by the cotton fields is killing the pests but the other animals that eat them are also being killed and it can affect the whole food chain.

Everyone is responsible

Everyone needs to be responsible for the use of technology because it affects everyone.

2 Chapter 2 chemistry of life

2.1 From atoms to molecules

Elements

Elements are basic building blocks of matter. (Matter is anything that takes up space and has mass). An element is classified by the number of atoms, protons, chemical reaction, and electrons. The human body has only 4 of the elements: Carbon, nitrogen, oxygen, and hydrogen. See the complete table below!

Atoms

An atom is the smallest unit of an element. With in the atom there are smaller sub atomic elements such as protons, neutron and electrons. The protons carry a positive charge and the electrons have a negative charge.

Isotopes

Isotopes of the same type of atom differ in the number of neutrons. When an atom is breaking down it gives of rays of energy this turns it into a radioisotope. There are two different levels of radiation that is given off. The lower level of radiation is considered a tracer. A tracer is used to image a body organ. The high levels of radiation can kill cells and damage DNA this can have long lasting affects.

Molecules and compounds

A molecule can only contain atoms of the same kind. When they are different is then put into another category: compound.

· Ionic bonding the outer shell contains 8 electrons. During the ionic reaction the atom will gain or lose electrons to stabilize itself. 8 electrons is the ideal number.
· Covalent bonding this means that two or more atoms can share an outer shells.

2.2 water and living things

Water makes up about 60-70% of the total body weight. The oxygen molecule is negative and the two hydrogen molecules attached to it are positive.

Hydrogen bonds

It is considered a hydrogen bond when the hydrogen molecule is positive attached to a negative atom.

Properties of water

1. Liquid
2. vaporization
3. frozen
4. cohesive
5. polar charged
Each of these is taken in place with water. Ions that interact with water are hydrophilic and the ones that don’t are hydrophobic.

Acids and bases

· Acidic solutions are High H+ this means that they release hydrogen ions when in water.
· Basic solutions are give of OH- hydroxide ions when put in water.
· The PH scale represents how acidic something is or if it’s a base. This ranges from 0-14.
· Buffers are what keeps the PH level, level.

2.3 Molecules of life

There are four categories of organic molecules these include carbs, lipids, proteins, and nucleic acids.

2.4 carbohydrates

Carbs are simple sugars and are used for energy.

2.5 Lipids

Lipids are for long term energy storage. Mammals and other vertebrates use them. Some examples of lipids fats which are found in animals typically. There are oils that typically come from different plants. There is a difference between saturated, unsaturated, and Trans fatty acids.
The saturated have no double bonds between the carbon atoms.
The unsaturated have the double bonds where ever there is hydrogen’s are less 2 per carbon.
Last but least the Trans fatty acids are vegetable oils that have been particularly hydrogenated to seem solid. All the double bonds are saturated.

Steroids

These are lipids that have entirely different structure from those of fats. They have 4 fused carbon rings.

2.6 proteins

Proteins are the primary function and structure of cells.

· Support
· Enzymes
· Transport
· Defense
· Hormones
· Motion

Shape of the proteins

Extreme heat will cause them to undergo denaturation.

Levels of protein

· The first level is the primary structure.
· The second is the secondary structure
· The third is the tertiary structure and this is the final 3-D shape.
· The four is the quaternary structure.

2.7 Nucleic Acids

This is the DNA and RNA. They were demeaned this because they were detected in the nucleus of the cells.

DNA

DNA contains sugar and also contains the bases of adenine, guanine, thymine, and cytosine. This also has a double stranded look

RNA
RNA contains a different type of sugar then the DNA and has different bases and is not a helix.

These are both used to store use the information. Components are nucleotides.

Chapter 3 Cell structure and function

3.1 What is a cell?

The cell theory

This is the basic unit of life. Every living thing in the world is made up of cells. And cells can only be recreated by other existing cells.

The Cell size

Depending on the cell you are looking at it could be visible by the naked eye but human cells are not. The larger the size of the cell the more nutrients need to feed it so it will thrive.

Microscopy and cell structure

Micrographics are photos that are taken by the compound light microscope. This is also the type of microscope that we used in out unit one lab. (Please see the lab below)

~ Microscope Lab~

The first microscope first came about into the world in 1595 thanks to one of the most brilliant men, Zacharies Janssen. Since he was so young at the time some say his father probably built the first one but Zach eventually took over the production. Two other men made great changes to the microscope and helping it are what it is today. The first was Hooke he vastly improved the image that is giving off from the microscope. Second was Van Leeuwenhoek he built the one lenses microscope. He was famous for more then just the microscope he also discovered the bacteria and helped prove the theory of blood circulation.

. There are four main microscopes in the world are:
· The Compound Light Microscope
· Dissection
· Scanning Electron Microscope (SEM)
· Transmission Electron Microscope (TEM)

These microscopes range in the quality to the price.

The most common being the compound light microscope and it also the cheapest one on the market. This offers a 2-D image and is light laminated.

Dissection microscope is also lit by a light but reveals a 3-D picture. This however has low magnification.

The SEM model is one of the more pricy ones but it does pay off. This offers a 3-D black and white high resolution and high magnification picture.

The most expensive is the TEM but gives off a 2-D high magnification and high resolution picture.

These are the parts of the Microscope:
Aperture diaphragm
This is the hole in the stage that houses the slide. This also lets the light shine through to be able to view the slide. This is one of the most critical pieces of the microscope!!!
Body Tube
This part supports the eyepiece and objectives. It is critical that the tube be constructed so that this optics shares a common axis
Coarse Adjustment Knob
This moves either the body tube, or the stage up or down in a quick manner. A good coarse focus control will provide smooth, back lash free movement. This is used for the initial focusing magnification. You would use this before the fine focus. The coarse adjustment knob shouldn’t be moved unless it is set on the smallest magnification. It could crack or damage the specimen.
Eyepiece
The eye piece is self explanatory but I will amuse you anyways. The eye piece is how you view the slide!
Fine focus knob
This control allows for precise focusing of the specimen. This is best done when looking the microscope at the slide.
Base
It rests on the bench top and supports the stage and body of the microscope and in many cases also holds the lamp.
Arm
The arm is attached to the foot and supports the body tube.
Internal lamp
The lamp is used to light the objects in the slide for better viewing of the different parts. With out this you could not see the slides very well or at all.
Nosepiece
This accommodates at least 4 different magnification objectives. On the microscope that we are using today will have 4x,10x,40x, and 100x. This can change the magnification of the slide, letting you see more or less.
Objective
The objective is the actual microscope. This will let you examine objects to at least 400x the size. With out this the microscope would be pointless! These can be changed safely by looking at the microscope. If done when looking in the microscope it could damage the slide if the objective hits it.
Stage clips
These are the basic stage slide holders. Supplied in pairs, they are adequate for general slide manipulation up to a maximum of 400x. They hold the slides in place. This can affect image immensely if they were not present your slide may not stay still where you place it.
Stage
This is the platform that supports the specimen (which are typically mounted on glass slides). To do this job properly it must be perfectly perpendicular to the optical axis, dead flat and of adequate size. This can be adjusted and viewed by looking at the Microscope.

The founding fathers of the microscope changed the world for ever we have accomplished so much that we never thought we would. Thanks to the vast improvements to the microscopes over the years we have been able to find out more about diseases and have been able to impregnate woman who never thought they would be able to have kids. We have giving children hope to live when they thought death is all that seeks them. We have fought AIDS, Cancer, and blood born diseases.

3.2 How are cells organized?

Evolutionary History of the animal cell

Prokaryotic cell lack a nucleus and has a membrane enclosed structure where the DNA is found. It is mainly represented by the bacteria and archaea. DNA is loose in the cytoplasm. The small ribosomes are assembled from the DNA information.

Eukaryotic cells in humans have a nucleus and have larger ribosomes. The eukaryotic cells do not have a membrane that surrounds but does carry cellular respiration.

3.3 The Plasma Membrane and how substances cross it

1. Diffusion
2. Osmosis
3. Facilitated Transport
4. Acetic Transport
5. Endocytosis and exocytosis
The plasma membrane is selectively permeable and only lets certain molecules to enter and exit. Diffusion also takes place in the membrane; diffusion is the random of molecules from a higher concentration to lower concentration. Then osmosis takes place during osmosis it is the diffusion of water molecules. Facilitated transport is the transport of molecules across the plasma membrane from higher concentration to concentration VIA a protein carrier. Acetic transport is the movement molecule from to higher using ATP as energy requires a protein carrier. Then exocytose transports molecules outside the cell VIA fusion of a versatile with the plasma membrane.

3.4 The nucleus and the production of proteins

The nucleus

The nucleus is the middle and most promenade structure in the cell. The nucleus is trapped within the nucleolus envelope.

Ribosomes

The ribosomes are made of organelles of protein and RNA. There are found in the endo plasma and free float in the cell.

The endo membrane system

The endomembrane system contains a nuclear envelope, vesicles, Golgi Lysosome, and endo plasmic reticulum.

3.5 The cytoskeleton and cell movement

Cilia and Flagella

They are both made of microtubules and both used in movement. One of the big differences is that cilia are about 20x the size of the flagella.

3.6 Mitochondria and cellular Metabolism

in the mitochondria there is am important function happening: Cellular Respiration. Cellular respiration produces ATP in a cell. ATP gives us the quick burst of energy that we need.

Chapter 4 Organization and regulation of body systems

There are 4 different types of tissue in the body:

4.1 Types of tissues

a tissue is composed of specialized cells of the same type that perform a common function in the body. The 4 types of tissues are:
1. Connective
2. Muscular
3. Nervous
4. Epithelial

4.2 connective tissue connects and supports

The connective tissue is responsible supporting and binding the body together. The three main types of tissues that make connective tissue up are:
1. Fibrous
2. supportive
3. fluid

These all play a special role in the body such as supporting the blood vessels, nerves, and the cell population.

4.3 Muscular tissue moves the body

The muscle is what allows a runner to run and a gymnast to do the rings. This is how we move. There are also three types of tissues that make it up:
1. Skeletal
2. Smooth
3. Cardiac
Muscular tissue makes up some of the most vital organs in the body such as your heart.

4.4 nervous tissues communicates

The nervous tissues are what communicate when something hurts or feels good. This is where the sensory impute lies. However this is a large system but is only made up of two major cells
1. neurons – carries messages
2. neuralgia – supports the neurons

4.5 epithelial tissues protects

This can include the lining of the lungs to lining of the nose

4.6 cell junctions

There are three different types of cell junctures:
1. Tight
2. adhesion
3. Gap

The tight junctures are the zipper like fasting between the cells. The adhesion junctions are what let the cells stretch and bend. The gap junctions allow the smaller molecules and signals to pass between the cells.

4.7 Integument system

This includes the organ systems and the skin. The skin is the most conspicuous system because it cover the outside of the body and is about 15% the weight of an adult. There are different regions of the skin
1. The epidermis is the very top layer of the skin. This is also where skin cancer can be seen.
2. The Dermis is the region of the dense fibrous connective tissue below the epidermis.
3. then the subcutaneous layer this not considered part of the skin but is the common site for injections.
The skin also contains the sweat glands, the hair follicles, oil glands, and the acne.

4.8 organ systems

Integumentary system
Protects the body
Receives sensory input
Helps control temperature
Synthesis vitamin D

2. Cardiovascular system
a. transports blood, nutrients, gases, and wastes.
b. defends against disease
c. helps control temperature, fluid, and pH balance
3. Lymphatic and immune system
a. Helps control fluid and balance
b. Absorbs fat
c. Defends against infectious disease
4. Digestive system
a. ingests food
b. Digests food
c. absorbs nutrients
d. eliminates waste
5. Respiratory system
a. maintains breathing
b. exchanges gases at lungs and tissues
c. helps control pH balance
6. Urinary system
a. excretes metabolic wastes
b. helps control fluid balance
c. helps control pH balance
7. Skeletal System
a. supports the body
b. protects the bodies parts
c. helps move the body
d. stores minerals
e. produces blood cells
8. Muscular system
a. maintains posture
b. moves body and internal organs
c. produces heat
9. Nervous system
a. receives sensory impute
b. integrates and stores input
c. initiates motor output
d. helps coordinate organ systems
10. Endocrine system
a. produces hormones
b. helps coordinate organ systems
c. responds to stress
d. helps regulate fluid and pH balance
e. Helps regulate metabolism
11. Reproductive system
a. produces gametes
b. transports gametes
c. produces sex hormones
d. nurtures and gives birth to offspring in females

4.9 homeostasis

is the relative constancy of the internal environment and all the organs help contribute to this.

These are the website where I got some of the pictures that helped me understand some of the material.

www.corrosionsource.com/.../periodic_table.gif
whyfiles.org/034clone/images/dna_molecule.gif
www.blackwellpublishing.com/.../Fig1-9.jpg
www.hometrainingtools.com/misc/compound%20par...
http://scienceblogs.com/clock/2006/11/cell_structure.php

Self Evaluation Unit One

1. What were the three aspects of the assignments I've submitted that I am most proud of?

I don’t have three of them but I did think that my cell was a neat idea because it was different then just drawing and a Jell-O mold. My DNA and RNA strands were also different but a lot of work.

2. What two aspects of my submitted assignments do I believe could have used some improvement?

I defiantly believe that my essays could have been a 100x better but I was under a time crunch do to moving so I am sure that they are the bare minimum that I need to pass with at least a "C".

3. What do I believe my overall grade should be for this unit?

I believe that I deserve a "C" because I could always do better. However I am hoping to walk out of this unit with a "B".

4. How could I perform better in the next unit?

I really needed to spend more time on this unit. So I will defiantly use my time more wisely and be sure not to put anything off I didn’t think that there was as much as there was.


1. At what moment during this unit did you feel most engaged with the course?

I Felt the most engaged in the unit when the different parts of the cells where being described, Even though it is a lot to remember and to think about. I was excited to do the cell lab project!

2. At what moment unit did you feel most distanced from the course?

I felt most distance from the course in chapter one I thought it was kind of boring to tell the truth. I think it is because I have already covered the stuff in chapter one in a different class so nothing was new to me. I was a little disappointed.

3. What action that anyone (teacher or student) took during this unit that find most affirming and helpful?

When I had questions about how to present the DNA and RNA in the cell lab project, Larry Frolich was there to help assure me that it was ok to be able to do a separate model of it. I felt like had let some of the stress off of me.

4. What action that anyone (teacher or student) took during this unit did you find most puzzling or confusing?

I truly don’t feel like and one person was the most confusing because i think everyone was lost of confused at one time and there is very little communication between different students. I believe that the next unit will be better because now we all know what to expect. I however have already started on the next unit because this unit I put off to long and now I have to struggle to get it in on time. I didn’t realize how much work it entailed.

5. What about this unit surprised you the most? (This could be something about your own reactions to the course, something that someone did, or anything else that occurs to you.)
I think I was completely shocked when i saw how much work was actually needed! I was moving at the same time as this project was do so I was without a computer for a week and it has made it extremely difficult so I hope to walk out with a "B" but next unit I will be getting an "A". Never settle for second best!!

Cell Lab Unit One



The materials used in making the cell:
Ø Large Floral Foam
Ø Small Floral Foam
Ø Light Blue Play dough
Ø Light Yellow Play dough
Ø Yellow Play dough
Ø Purple Play dough
Ø Light Purple Play dough
Ø Blue Play dough
Ø Green Play dough
Ø Toothpicks
Ø Paper


This is a cell that is showing:
The Plasma Membrane, which is located on the outside of the cell. The Plasma Membrane has an important role, it decides what molecules will enter and exit the cell and what will not. It is made up proteins and phospholipids. This is the blue play dough on the outside.
Cytoskeleton maintains the cells shape and assists movement of the cell parts.
With in the cytoskeleton:
· Cytoplasm and in my model it is represented with the light yellow play dough. It is a semi fluid matrix surrounding the nucleus and it holds the organelles.
· Microtubules these are cylinders of protein molecules. Centrioles, cilia, and flagella. (shown by the thin pink strands)
· Intermediate filaments are protein fibers that give the cell strength and support. (Shown in green strands)
· Actin filaments help in the movements with the cell and with the organelles with in the cell. (Shown in the think purple strands)
· Lysosome is a vesicle that digests the macromolecules and even out the cell parts.
· Vesicle is a membrane bound sac that stores and transports substances.
Nucleus stores genetic information.
· Nuclear envelope double membrane with nuclear pores the covers the nucleus
· Chromatin the threads shown with the dark purple. These threads containing DNA and protein
· Nucleolus shown in the dark purple ball. This region produces sub units of ribosomes.
Endoplasmic Reticulum:
· Rough ER has the yellow balls that represent the rough edge.
· Smooth ER does not have the yellow balls but the smooth synthesizes lipid molecules
· Mitochondrion is the pink bean looking organelle on the cell. This carries out cellular respiration producing ATP molecules. This is thought to be derived from an engulfed prokaryotic cell.
· The Polyribosome is the yellow tail thing and it is a string of ribosomes synthesizing the same protein.
· Last but not least the Golgi Apparatus this processes, packages, and secretes modified cell products.This is the RNA strandThis is the DNA strand

The replication of the DNA can be described it 5 steps: (pg 445)
1. There are two strands that were part of the parental DNA are hydrogen-bonded together.
2. An enzyme unzips the double stranded DNA
3. Complementary DNA nucleotides are fit into place. This is possible with the enzyme DNA polymerase.
4. An enzyme then seals any breaks in the sugar phosphate backbone
5. After this has all taken place then you will have two new strands of DNA that are exact copies of the parent DNA strand.
What did I learn?
I learned the different parts of the Cell and how it is formed including how DNA is made. I am a hands on learner, so building the cell really helped me understand the cell.

Did I have fun?
Some parts I found fun but others I found boring but the actual model was fun. I don’t think I have played with play dough since I was a little girl! It really brought me back to my child hood in an adult kind of way!

Microscope Lab Unit One

This is the cheek smear at 100x

This is the bacteria capsual at 40x



This is the Onion Root Tip at 10x



This is the letter "E" under 4x

The first microscope first came about into the world in 1595 thanks to one of the most brilliant men, Zacharies Janssen. Since he was so young at the time some say his father probably built the first one but Zach eventually took over the production. Two other men made great changes to the microscope and helping it are what it is today. The first was Hooke he vastly improved the image that is giving off from the microscope. Second was Van Leeuwenhoek he built the one lenses microscope. He was famous for more then just the microscope he also discovered the bacteria and helped prove the theory of blood circulation.

. There are four main microscopes in the world are:
· The Compound Light Microscope
· Dissection
· Scanning Electron Microscope (SEM)
· Transmission Electron Microscope (TEM)

These microscopes range in the quality to the price.

The most common being the compound light microscope and it also the cheapest one on the market. This offers a 2-D image and is light laminated.

Dissection microscope is also lit by a light but reveals a 3-D picture. This however has low magnification.

The SEM model is one of the more pricy ones but it does pay off. This offers a 3-D black and white high resolution and high magnification picture.

The most expensive is the TEM but gives off a 2-D high magnification and high resolution picture.

For our lab today we will be demonstrating the Compound Light Microscope:

These are the parts of the Microscope:


Aperture diaphragm

This is the hole in the stage that houses the slide. This also lets the light shine through to be able to view the slide. This is one of the most critical pieces of the microscope!!!
Body Tube
This part supports the eyepiece and objectives. It is critical that the tube be constructed so that this optics shares a common axis
Coarse Adjustment Knob

This moves either the body tube, or the stage up or down in a quick manner. A good coarse focus control will provide smooth, back lash free movement. This is used for the initial focusing magnification. You would use this before the fine focus. The coarse adjustment knob shouldn’t be moved unless it is set on the smallest magnification. It could crack or damage the specimen.
Eyepiece
The eye piece is self explanatory but I will amuse you anyways. The eye piece is how you view the slide!
Fine focus knob
This control allows for precise focusing of the specimen. This is best done when looking the microscope at the slide.
Base
It rests on the bench top and supports the stage and body of the microscope and in many cases also holds the lamp.
Arm
The arm is attached to the foot and supports the body tube.
Internal lamp
The lamp is used to light the objects in the slide for better viewing of the different parts. With out this you could not see the slides very well or at all.
Nosepiece
This accommodates at least 4 different magnification objectives. On the microscope that we are using today will have 4x,10x,40x, and 100x. This can change the magnification of the slide, letting you see more or less.
Objective
The objective is the actual microscope. This will let you examine objects to at least 400x the size. With out this the microscope would be pointless! These can be changed safely by looking at the microscope. If done when looking in the microscope it could damage the slide if the objective hits it.
Stage clips

These are the basic stage slide holders. Supplied in pairs, they are adequate for general slide manipulation up to a maximum of 400x. They hold the slides in place. This can affect image immensely if they were not present your slide may not stay still where you place it.

Stage
This is the platform that supports the specimen (which are typically mounted on glass slides). To do this job properly it must be perfectly perpendicular to the optical axis, dead flat and of adequate size. This can be adjusted and viewed by looking at the Microscope.

These are Cancer Cells This is the HIV/AIDS

The founding fathers of the microscope changed the world for ever we have accomplished so much that we never thought we would. Thanks to the vast improvements to the microscopes over the years we have been able to find out more about diseases and have been able to impregnate woman who never thought they would be able to have kids. We have giving children hope to live when they thought death is all that seeks them. We have fought AIDS, Cancer, and blood born diseases.